transgenicanimalsorRNAitechniques.Majorityoftargetsmeetsomebutnotall
AntibodySequencing:GenScript'sadvancedAntibodySequencingtechnologyoffersfastandprofessionalsequencingservicesforyourmonoclonalantibodies.
TargetIdentificationandValidation
PK/PDStudy:GenScriptoffersover120tumorandinflammationmodelsforevaluationofinvivoefficacy,PK/PD,biomarkerandbioanalysisstudies.GenScriptAnti-idiotypeAntibodyservicesarealsoapowerfultoolforantibodydrugPK/PDandimmunogenicitystudies.
definedashavinggatheredadequatescientificevidenceforthetarget'sdisease
Deletionofthemouseorthologand/oroverexpressionofmouseproteinshouldmimichumanpathology1.
AntibodyDiscovery:GenScript'sAntibodyEngineeringgroupcanbuildantibodylibrarywithupto1010individualclones,tospeedupyourantibodydiscoveryefforts.
transgenicanimalsandcelllines.Biologicalexperimentsalsoincludeknockout,
"Omic"studies:Transcriptionalprofilingandproteomicsdatathatleadtothediscoveryofgenesandproteinswithaberrantexpressionpatternsindiseasestates.
GenScriptAntibodyDrugDiscoveryServices
资源»
TargetAssessment&Validation
Assays:GenScripthasdevelopedseveralcell-basedADCC/CDCfunctionalassaystoprofiletheefficacyandpotencyofyourtherapeuticantibodiesusingproprietaryrecombinanteffectorcells.
AntibodyEngineering:GenScriptscientists'extensiveexperienceinantibodyengineeringcanprovidesuperiorservicessuchasantibodyhumanization,affinitymaturationandmore.AntibodyProduction:Withsolidexpertiseinrecombinantantibody(rAb)productiontechniques,GenScriptprovidesacomprehensiverAbserviceportfoliothatdelivermicrogramtogramquantitiesofpurerAbforeachstageofyourAbdrugdiscoveryprogram.
References
Therapeuticstrategyisdefined:Forexample,acommonmolecularmechanismforanantibody-basedtherapeuticistheblockadeofaligand-receptorinteraction,forwhichtherearethreeconceivabletargetingstrategies:ananti-ligandantibody,ananti-receptorantibody,andareceptor-Fcfusionprotein1.
Ab-basedinterventionoftargetinananimalmimickinghumandiseaseshouldyieldthedesiredtherapeuticoutcome1.
Typicallyamonoclonalantibody(mAb)recognizingthemouseorthologofthe
Whataresomeoftheconsiderationsthatgointotargetselection?
criteriaandadditionalvalidationeffortsareoftenconducted.
Researchmaterials/reagentsatthisstagetypicallyincludesurrogateantibodies,
YoucanalsoviewourRecombinantAntibodyServiceSelectionGuidetoidentifyservicesthatarethebestmatchforyourapplication.
Buthowisatargetselected?
生物药技艺地方»
AssessmentandValidation
TargetIdentification,
Expression:Shouldbeexpressedinpathologicaltissue.
Concentration:ShouldbepresentatlevelthatisdetectableandcanbestoichiometricallyboundbyAbtherapeutic.Whathappensafteratargetisidentified?
邮箱:bioassay@genscript.com.cn;
Targetaccessibility:ItshouldresideinphysiologicallocationaccessibletoAb.BraintargetsarenotoriouslydifficulttotreatusingAbtherapeuticsbecausetheblood-brainbarrierpreventstheirentryintobrain.SingledomainAbs/nanobodiescanhelpinsuchcasesowingtotheirsmallsize(~13kDa).
1Shih,H.H.inDevelopmentofAntibody-BasedTherapeutics(edM.A;BornsteinTabrizi,G.G.;Klakamp,S.L.)Ch.2,9-32(Springer,2012).
TargetidentificationinAntibodyDrugDiscoveryistheprocessofidentifyingthemoleculartargetofanAntibody-usuallytargetcellsurfacemoleculessuchasreceptorsorantigensexpressedontumorcellsorotherhostcells.Stepsinvolveidentifyingdruggabletargets(definedasthelikelihoodofbeingabletomodulateatargetwithatherapeutic).Targetidentificationcanbeapproachedbydirectbiochemicalmethods,geneticinteractionsorcomputationalinference.Inmanycases,however,combinationsofapproachesmayberequiredtofullycharacterizeon-targetandoff-targeteffectsandtounderstandmechanismsofAb-bindingtothetargetantigen.
studies,includingmousegenetics.Exampleslistedbelow:
Theprevioussectioninthisseriesis"AntibodyDrugDiscoveryOverview".Toreview,click here.
电话:400-025-8686-5809
技艺资源地方»
TargetIdentification
intendedhumanproteinisobtainedasasurrogateAbtofacilitatetargetvalidation.
associationanditstherapeuticpotential.Thisisachievablethroughanimalmodel
Basedonpublisheddata:Literaturetarget,patentsetc.
Thefirststepafteratargethasbeenidentifiedistovalidateit.Validationissimply
