TargetIdentification,
Concentration:ShouldbepresentatlevelthatisdetectableandcanbestoichiometricallyboundbyAbtherapeutic.Whathappensafteratargetisidentified?
AssessmentandValidation
技艺资源地方»
AntibodySequencing:GenScript'sadvancedAntibodySequencingtechnologyoffersfastandprofessionalsequencingservicesforyourmonoclonalantibodies.
studies,includingmousegenetics.Exampleslistedbelow:
Researchmaterials/reagentsatthisstagetypicallyincludesurrogateantibodies,
Targetaccessibility:ItshouldresideinphysiologicallocationaccessibletoAb.BraintargetsarenotoriouslydifficulttotreatusingAbtherapeuticsbecausetheblood-brainbarrierpreventstheirentryintobrain.SingledomainAbs/nanobodiescanhelpinsuchcasesowingtotheirsmallsize(~13kDa).
生物药技艺地方»
AntibodyDiscovery:GenScript'sAntibodyEngineeringgroupcanbuildantibodylibrarywithupto1010individualclones,tospeedupyourantibodydiscoveryefforts.
TargetidentificationinAntibodyDrugDiscoveryistheprocessofidentifyingthemoleculartargetofanAntibody-usuallytargetcellsurfacemoleculessuchasreceptorsorantigensexpressedontumorcellsorotherhostcells.Stepsinvolveidentifyingdruggabletargets(definedasthelikelihoodofbeingabletomodulateatargetwithatherapeutic).Targetidentificationcanbeapproachedbydirectbiochemicalmethods,geneticinteractionsorcomputationalinference.Inmanycases,however,combinationsofapproachesmayberequiredtofullycharacterizeon-targetandoff-targeteffectsandtounderstandmechanismsofAb-bindingtothetargetantigen.
Therapeuticstrategyisdefined:Forexample,acommonmolecularmechanismforanantibody-basedtherapeuticistheblockadeofaligand-receptorinteraction,forwhichtherearethreeconceivabletargetingstrategies:ananti-ligandantibody,ananti-receptorantibody,andareceptor-Fcfusionprotein1.
邮箱:bioassay@genscript.com.cn;
References
Deletionofthemouseorthologand/oroverexpressionofmouseproteinshouldmimichumanpathology1.
associationanditstherapeuticpotential.Thisisachievablethroughanimalmodel
资源»
Theprevioussectioninthisseriesis"AntibodyDrugDiscoveryOverview".Toreview,click here.
transgenicanimalsorRNAitechniques.Majorityoftargetsmeetsomebutnotall
PK/PDStudy:GenScriptoffersover120tumorandinflammationmodelsforevaluationofinvivoefficacy,PK/PD,biomarkerandbioanalysisstudies.GenScriptAnti-idiotypeAntibodyservicesarealsoapowerfultoolforantibodydrugPK/PDandimmunogenicitystudies.
AntibodyEngineering:GenScriptscientists'extensiveexperienceinantibodyengineeringcanprovidesuperiorservicessuchasantibodyhumanization,affinitymaturationandmore.AntibodyProduction:Withsolidexpertiseinrecombinantantibody(rAb)productiontechniques,GenScriptprovidesacomprehensiverAbserviceportfoliothatdelivermicrogramtogramquantitiesofpurerAbforeachstageofyourAbdrugdiscoveryprogram.
TargetIdentification
GenScriptAntibodyDrugDiscoveryServices
Typicallyamonoclonalantibody(mAb)recognizingthemouseorthologofthe
Expression:Shouldbeexpressedinpathologicaltissue.
"Omic"studies:Transcriptionalprofilingandproteomicsdatathatleadtothediscoveryofgenesandproteinswithaberrantexpressionpatternsindiseasestates.
YoucanalsoviewourRecombinantAntibodyServiceSelectionGuidetoidentifyservicesthatarethebestmatchforyourapplication.
Thefirststepafteratargethasbeenidentifiedistovalidateit.Validationissimply
criteriaandadditionalvalidationeffortsareoftenconducted.
TargetAssessment&Validation
intendedhumanproteinisobtainedasasurrogateAbtofacilitatetargetvalidation.
Whataresomeoftheconsiderationsthatgointotargetselection?
Assays:GenScripthasdevelopedseveralcell-basedADCC/CDCfunctionalassaystoprofiletheefficacyandpotencyofyourtherapeuticantibodiesusingproprietaryrecombinanteffectorcells.
definedashavinggatheredadequatescientificevidenceforthetarget'sdisease
TargetIdentificationandValidation
Basedonpublisheddata:Literaturetarget,patentsetc.
电话:400-025-8686-5809
transgenicanimalsandcelllines.Biologicalexperimentsalsoincludeknockout,
1Shih,H.H.inDevelopmentofAntibody-BasedTherapeutics(edM.A;BornsteinTabrizi,G.G.;Klakamp,S.L.)Ch.2,9-32(Springer,2012).
Ab-basedinterventionoftargetinananimalmimickinghumandiseaseshouldyieldthedesiredtherapeuticoutcome1.
Buthowisatargetselected?
