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GAPDH Antibody, pAb, Goat
*This product is under development*
| Synonyms | Anti-GAPDH; |
| Description | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often used as a control in experiments quantifying specific changes in expression levels of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes, such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. GenScript Goat Anti-GAPDH Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography. |
| Host Species | Goat |
| Conjugation | Unconjugated |
| Immunogen | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle |
| Purification | Immunoaffinity chromatography |
| Subclass | Goat IgG |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | GenScript Goat Anti-GAPDH Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species. |
| Species Reactivity | Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish and E. coli |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Other applications: user-optimized |
Xia H.,et al. MiR-214 targets β-catenin pathway to suppress invasion, stem-like traits and recurrence of human hepatocellular carcinoma.PLoS One.2012Sep;7(9):e44206
C Cao.,et al. Hypertonic Saline Reduces Lipopolysaccharide-Induced Mouse Brain Edema Through Inhibiting Aquaporin 4 Expression.Crit Care.2012Otc;16(5):R186
Andrea R. Beyer.,et al. Nucleolar Trafficking of the Mouse Mammary Tumor Virus Gag Protein Induced by Interaction with Ribosomal Protein L9.J Virol.2013Jan;87(2):1069 - 1082
Zhang L.,et al. GLPâ€1 analogue prevents NAFLD in ApoE KO mice with diet and Acrp30 knockdown by inhibiting câ€JNK.Liver Int.2013May;33(5):794 - 804
Cao JX.,et al. Activation of caspase-3 and its correlation with shear force in bovine skeletal muscles during postmortem conditioning.J Anim Sci.2013Sep;91(9):4547-52
Winyoo Chowanadisai.,et al. Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12).Proc Natl Acad Sci U S A.2013May;
Hu M.,et al. Over-expression of regulator of G protein signaling 5 promotes tumor metastasis by inducing epithelial–mesenchymal transition in hepatocellular carcinoma cells.J Surg Oncol.2013Jul;
Guo Q.,et al. PAK4 kinase-mediated SCG10 phosphorylation involved in gastric cancer metastasis.Oncogene.2013Aug;
Kumari R.,et al. Agonist-dependent signaling by group I metabotropic glutamate receptors is regulated by association with lipid domains.J Biol Chem.2013Nov;
Cao J.,et al. Activation of caspase-9 and its influencing factors in beef during conditioningAnimal.2013Dec;
请点击此处查找更多相关文献
C Cao.,et al. Hypertonic Saline Reduces Lipopolysaccharide-Induced Mouse Brain Edema Through Inhibiting Aquaporin 4 Expression.Crit Care.2012Otc;16(5):R186
Andrea R. Beyer.,et al. Nucleolar Trafficking of the Mouse Mammary Tumor Virus Gag Protein Induced by Interaction with Ribosomal Protein L9.J Virol.2013Jan;87(2):1069 - 1082
Zhang L.,et al. GLPâ€1 analogue prevents NAFLD in ApoE KO mice with diet and Acrp30 knockdown by inhibiting câ€JNK.Liver Int.2013May;33(5):794 - 804
Cao JX.,et al. Activation of caspase-3 and its correlation with shear force in bovine skeletal muscles during postmortem conditioning.J Anim Sci.2013Sep;91(9):4547-52
Winyoo Chowanadisai.,et al. Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12).Proc Natl Acad Sci U S A.2013May;
Hu M.,et al. Over-expression of regulator of G protein signaling 5 promotes tumor metastasis by inducing epithelial–mesenchymal transition in hepatocellular carcinoma cells.J Surg Oncol.2013Jul;
Guo Q.,et al. PAK4 kinase-mediated SCG10 phosphorylation involved in gastric cancer metastasis.Oncogene.2013Aug;
Kumari R.,et al. Agonist-dependent signaling by group I metabotropic glutamate receptors is regulated by association with lipid domains.J Biol Chem.2013Nov;
Cao J.,et al. Activation of caspase-9 and its influencing factors in beef during conditioningAnimal.2013Dec;
请点击此处查找更多相关文献
