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THE™ DYKDDDDK Tag Antibody, mAb, Mouse
| Synonyms | Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb; |
| Description | The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THETM DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide (DYKDDDDK) coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
| Purification | Protein A affinity column |
| Subclass | IgG2b, k |
| Clone ID | 5A8E5 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product per customer's reuqest including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Immunoprecipitation: 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry: 1 µg/ml Other applications: user-optimized |
Reuben A, et al. The WT hemochromatosis protein HFE inhibits CD8 T‐lymphocyte activation. Eur J Immunol. 2014Mar.;
Yan-Jhu Peng.,et al. A RINGâ€type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis.Plant J.2013Apr;74(2):310-27
Ivy KD.,et al. A Re-Evaluation of the Role of hCTR1, the Human High-Affinity Copper Transporter, in Platinum-Drug Entry into Human Cells.Mol Pharmacol.2013Jun;83(6):1237 - 1246\x09\x09\x09
Maryon EB.,et al. Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1.Am J Physiol Cell Physiol.2013Apr;304(8):C768 - C779
Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;
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Yan-Jhu Peng.,et al. A RINGâ€type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis.Plant J.2013Apr;74(2):310-27
Ivy KD.,et al. A Re-Evaluation of the Role of hCTR1, the Human High-Affinity Copper Transporter, in Platinum-Drug Entry into Human Cells.Mol Pharmacol.2013Jun;83(6):1237 - 1246\x09\x09\x09
Maryon EB.,et al. Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1.Am J Physiol Cell Physiol.2013Apr;304(8):C768 - C779
Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;
请点击此处查找更多相关文献
| 产品编号: A00187 | ||
|---|---|---|
规格 |
价格 |
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| 100 ug | ¥1000 | |
