Independent Antibody Validation
Epitope Binning is a means of antibody validation in which monoclonal antibodies are tested against one another to determine which antibodies compete for the same antigenic epitope. Antibodies which bind to identical epitopes are grouped together into "bins". Antibodies which are grouped into different bins are considered to be independent from one another, hence the name independent antibody validation (Figure 1). Independent antibody validation is used to identify which antibody pairs have higher specificity and/or sensitivity in 亚博的官网地址ious experimental platforms.
Figure 1. Independent Antibody Pairs. (A) An antigen can have multiple epitopes in which an antibody can bind. (B) If two mAbs bind to same epitope on an antigen, they are considered to be in one bin. (C) However, if two mAbs both bind to the same antigen, but at different epitopes, they are considered to be in separate bins.
Key Features of GenScript's Independent Antibody Validation Service
- Final Delivery: An epitope binning test report identifying all "independent pairs"
- Can be added to custom mAb generation services including Lead generation and optimization
- Can be performed on mAb's generated against protein antigens
- Can be performed on up to 5 mAbs at one time
- Can be performed between different groups of 5 mAbs
|Percent binding||mAb I-biotin||mAb II-biotin||mAb III-biotin||mAb IV-biotin|
|No competing Ab||100||100||100||100|
|mAb I competing||10.3||8.8||18.6||142.7|
|mAb II competing||7.6||7.7||8.6||134.8|
|mAb III competing||10.1||9.2||11.2||132|
|mAb IV competing||93.2||106.2||108||19.8|
Table 1. Example of Epitope Binning Report. The example epitope binning report contains the percentage of testing mAb which binds to a specific epitope when a competing mAb is present. The above table indicates that mAb I, mAb II, and mAb III belong to the same bin and are independent from mAb IV.
Interested in Learning More About GenScript's Custom mAb Generation Services Perfect for Independent Antibody validation?
Want to Try a Non-rodent mAb? Learn more About Our MonoRab™ Rabbit mAb Generation Service Which Includes Independent Antibody Validation for Free!
Increase mAb Specificity with GenScripts Premier Rabbit mAb Generation Service.
Perfect fit for Anti-ID and IVD Applications!
Unsure which service line is approproate for your application? Try our Antibody Service Selecting Tool.
Quotations and Ordering
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.