=
FormulasforDNAMolarConversions
centi
Recommended%Acrylamide
MolecularWeight
NucleicAcid
1µgofpBR322DNA
MetricPrefixes
→
µg×106pg/1µg×pmol/330pg×1/N=pmol
3.2×107
OptimumGeneCodonOptimization
≈
剖析生物学资源地方»
1pmolofpBR322DNA
50kDaprotein
ProteinMolarConversions
→
10-3
100pmolof100kDaprotein
1.0×106
1kbDNA
1A260unitofdouble-strandedDNA=50µg/ml
2.7kbDNA
Length/M.W.ofCommonNucleicAcids.
1µgof1,000bpDNA
5SrRNA(E.coli)
10-2
2.0
≈
≈
1µg
FormulasforDNAMolarConversions
pico
4.1×104
Protein/DNAConversions
nano
18SrRNA
pmol×N×660pg/pmol×1µg/106pg=µg
10-9
averageMWofanaminoacid
1A260unitofsingle-strandedRNA=40µg/ml
zepto
whereNisthenumberofnucleotidesand330pg/pmolistheaverageMWofanucleotide
NumberofNucleotides
资源»
PolyacrylamideGel(%):ResolutionofProtein
5.1×105
µ
48,502(dsDNA)
5µg
Toconvertpmoltoµg:
SpectrophotometricConversions
100pmolof1kDaprotein
103
0.7
m
1.2
2.AverageMWofssDNAbase=330.
50-2,000
8
Recommended%Agarose
Standards
DNAMolarConversions
100kDaprotein
ProteinMolarConversions
40-200kDa
=
Dalton(Da)isanalternatenamefortheatomicmassunit,andkilodalton(kDa)is1,000daltons.Thusaproteinwithamassof64kDahasamolecularweightof64,000gramspermole
1.AverageMWofdsDNAbasepair=600.
=
37kDaprotein
1A260unitofsingle-strandedDNA=33µg/ml
270bDNA
1,500
→
800-12,000
→
c
→
16SrRNA(E.coli)
pBR322DNA
12
21-100kDa
28SrRNA
=
atto
**Thiswebpageprovidesseveralusefultablesofcommonbiologicalconstantsandconversions.Theseincludemetricprefixes,spectrophotometiricconversions,agarose/polyacrylamidegelresolutions,etc.YoucanusethesetablestoaidyourDNA/RNA/proteinquantification,electrophoresisgelpreparation,proteinsynthesisandotherresearchactivities.
1.52pmol(3.03pmolofends)
pBR322DNA:（4,361bp）
Prefix
10-18
10-21
Symbol
pmol×N×330pg/pmol×1µg/106pg=µg
µg×106pg/1µg×pmol/660pg×1/N=pmol
AgaroseGel(%):ResolutionofLinearDNA
a
→
Factor
1.5
NucleicAcidsandProteinCalculations
75
ProteinSizeRange
333aminoacids
Toconvertµgtopmol:
p
micro
0.66µg
OptimumResolutionforLinearDNA
AgaroseGel(%):ResolutionofLinearDNA
z
100pmolof50kDaprotein
10µg
6.5×105
Length/M.W.ofCommonNucleicAcids.
femto
23SrRNA(E.coli)
1,000-30,000
Standards
*Molecularweightsbasedonactualsequence.
1.6×106
1.0
2,900
4,361(dsDNA)
2.8µg
ForssDNA
SpectrophotometricConversions
0.36pmolDNA
810bDNA
Toconvertµgtopmol:
30kDaprotein
FordsDNA
200-3,000
400-7,000
LearnMore
tRNA(E.coli)
100pmolof10kDaprotein
Toconvertpmoltoµg:
lambdaDNA
10-6
PolyacrylamideGel(%):ResolutionofProtein
10
120
100ng
4,800
k
10-15
技艺资源地方»
NucleicAcidsandProteinCalculationsSpectrophotometricConversions
110daltons
f
3.AverageMWofRNAbase=340.
0.5
500-10,000
Protein/DNAConversions
milli
10-40kDa
DNAMolarConversions
≈
MetricPrefixes
10-12
2.8×106
10kDaprotein
≈
1.35kbDNA
1,900
kilo
n
ForGeneSynthesis
(Sizeoffragmentsinnucleotides;bp)
2.5×104
1pmolof1,000bpDNA
