(x106cells/mL)
0.25mL
ApplicationnotesusingGenScript’sMagneticBeads
0mL
Density
Mediumvolume(CHOcells)
MagneticBeadsvolume
50mL
3.7
6.12
7.16
3.44
97.70%
91.82%
50mL
Beforeaddingmagneticbeads
113rpm
6.36
100rpm
100rpm
0%
96.36%
6.12
Density
2.64
6.08
2.5mL
100rpm
产品资源地方»
speed
5%
0.50%
资源»
Savetimeonpurification
96.30%
50mL
98%
1.25mL
100rpm
50mL
50mL
113rpm
2.97
byaddingmagneticbeadsdirectlytoyourcellculture
50mL
97.40%
ApplicationNotesUsingGenScript's
2.50%
ProteinAmagneticbeadscanbeusedtocaptureantibodiesfromcellcultures.Addingthemagneticbeadsdirectlyintothecellculturesandleavingthemshakingovernightsavestimeandsimplifiesthecaptureprocedure.Butdotheshakingmagneticbeadsharmthecells?WetestedthisbytestingtheviabilityofCHOcellcultureafteranovernightincubationwithAmMag™proteinAmagneticbeads.Wefoundthattheviabilityofthecellsisnotsignificantlyreduced.
Viability(%)
3.66
2.5mL
(x106cells/mL)
1.25mL
113rpm
97.50%
SeeData
0%
6.12
5%
2.50%
87.5%
100%
0mL
MagneticBeads
Viability(%)
96.76%
100%
2.69
4.5
96.40%
2.92
97.80%
113rpm
3.78
97.70%
0.50%
Magneticbeads/Culture(v/v)
50mL
500mL
Afteraddingmagneticbeads
50mL
6.12
Cultureflask
250mL
100%
100%
0.25mL
技艺资源地方»
