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目录产品 » 抗体产品 » 标签抗体 » THETM BrdU Antibody, mAb, Mouse

THE™ BrdU Antibody, mAb, Mouse

Synonyms Bromodeoxyuridine Antibody, mAb, Mouse; Bromo-deoxyuridine Antibody, mAb, Mouse
Description Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.
GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Immunogen BrdU conjugated to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1, κ
Clone ID 3E2D3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T).
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.015- 0.05 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other applications: user-optimized




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产品编号: A01650
规格
价格
3176 A01650-100 100 ug ¥1200