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目录产品 » 抗体产品 » 标签抗体 » TF-tag Antibody, pAb, Rabbit

TF-tag Antibody, pAb, Rabbit

Synonyms Rabbit Anti-TF-tag pAb;
Description Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in 亚博的官网地址ious systems.
Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins.

GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Purified recombinant TF protein
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized


Lin KF., et al. Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment. Biophys J. 2012 Jun;102(12):2818-27.

Eunyong Park and Tom A. Rapoport. Bacterial Protein Translocation Requires Only One Copy Of The Secy Complex In Vivo. J Cell Biol. 2012 Sep;198(5):881 - 893.

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产品编号: A01329
规格
价格
2674 A01329-40 40 ug ¥990