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目录产品 » 抗体产品 » 标签抗体 » GFP Antibody, pAb, Rabbit

GFP Antibody, pAb, Rabbit

Synonyms Rabbit Anti-GFP pAb;
Description Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other 亚博的官网地址iants.

GenScript Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Purified recombinant full-length GFP protein
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other 亚博的官网地址iants of GFP, such as CFP, YFP, eGFP and GFPuv.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized


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产品编号: A01388
规格
价格
2741 A01388-40 40 ug ¥990