|Synonyms||THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse;|
|Description||Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. Strep II tag (NWSHPQFEK) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
GenScript THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THETM NWSHPQFEK Tag Antibody (A01732) conjugated with FITC. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
|Immunogen||NWSHPQFEK peptide conjugated to KLH|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.|
|Specificity||THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
FACS: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml