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目录产品 » 抗体产品 » THE金牌抗体 » THETM ADP Antibody, mAb, Mouse

THE™ ADP Antibody, mAb, Mouse

Synonyms ADP Antibody, mAb, Mouse
Description Adenosine diphosphate (ADP) is the product of adenosine triphosphate (ATP) dephosphorylation by ATPases. It is an important intermediate in cellular metabolism as the partially dephosphorylated form of ATP. ADP is 5′-adenylic acid with an additional phosphate group attached through a pyrophosphate bond. Its molecular formula is C10H15N5O10P2 with the molecular weight of 427.201 g/mol. ADP immunoassay is a useful method for screening the activity of kinases and other ATPases. An ADP antibody is a key reagent for ADP immunoassays such as ELISA and TR-FRET.
GenScript THE™ ADP Antibody, mAb, Mouse is produced from the hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with ADP conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Immunogen ADP conjugated to KLH
Purification Protein A affinity column

Subclass IgG1,κ
Clone ID 9B2B9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/ml.
Specificity The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding (EC50). The cross-reactivity data obtained in a competitive ELISA system is as follows:

Compound

Cross reactivity with ADP%

ADP

100

GDP

53.33

ATP

7.11

GTP

4.37

cAMP

0.47

CTP

0.03

cGMP

0.01

GMP

0.0085

cIMP

0.005

AMP

< 0.0001

UMP

< 0.0001

cTMP

< 0.0001



Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Competitive ELISA: 0.001-0.01 µg/ml.
Other applications: user-optimized




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产品编号: A01799
规格
价格
1136749 A01799-100 100 ug ¥2500