|Synonyms||Rabbit Anti-Ty1-tag pAb;|
|Description||Well-characterized antibodies for epitope tags are common in the study of protein expression in 亚博的官网地址ious systems.
GenScript Rabbit Anti- Ty1-tag Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
|Immunogen||Ty1-tag epitope peptide conjugated to KLH|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||This Antibody recognizes both Ty1 tagged fusion proteins.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-2 µg/ml
Immunoprecipitation (IP): 2-5 µg/ml
Other applications: user-optimized