|Synonyms||Rabbit Anti-E-tag; Anti-E-tag|
|Description||Rabbit Anti-E-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1.0 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.|
|Immunogen||E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH|
|Concentration||1.0 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide|
|Specificity||This Antibody recognizes E tagged fusion proteins.|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized