Synonyms | THE™ β-Actin Antibody [Biotin], mAb, Mouse; |
Description | GenScript THETM β-actin Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM β-Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein A affinity column. |
Host Species | Mouse |
Conjugation | Biotin |
Immunogen | KLH-coupled synthetic peptide within residues 1-50 of human β-actin (Swiss Prot: P60709) |
Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
Subclass | Mouse IgG2a, k |
Clone ID | 2D1D10 |
Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
Reconstitution | Reconstitute the lyophilized antibody (100 μg) with 200 μl of deionized water (or equivalent) to the final concentration of 0.5 mg/ml. |
Specificity | GenScript THETM β-actin Antibody[Biotin], mAb, Mouse recognizes Mouse, Rabbit, Chicken, Human, Hamster, Cow, Goat, Fish, Pig, and Monkey. The antibody has not yet been trested in other species. |
Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA:0.05-0.2 µg/ml Western blot: 0.1-1 µg/ml Other applications: user-optimized |