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目录产品 » 抗体产品 » 标签抗体 » THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Synonyms THETM NWSHPQFEK Tag Antibody,mAb,Mouse
Description Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
Host Species Mouse
Conjugation Unconjugated
Immunogen NWSHPQFEK peptide conjugated to KLH
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Purification Protein A affinity column

Subclass IgG1 Κ
Clone ID 5A9F9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/ml
Immunoprecipitation: 1-5 µg/ml
ICC/IF: 1-3 µg/ml
ELISA: 0.001-0.002 µg/ml
Flow cytometry: 1 µg/ml


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Piotr Koprowski., et al. Genetic Screen for Potassium Leaky Small Mechanosensitive Channels (MscS) in Escherichia coli : RECOGNITION OF CYTOPLASMIC β DOMAIN AS A NEW GATING ELEMENT. J Biol Chem. 2011 Jan;286(1):877 - 888.

Paul Dominic B. Olinares., et al. Subunit Stoichiometry, Evolution, and Functional Implications of an Asymmetric Plant Plastid ClpP/R Protease Complex in Arabidopsis . Plant Cell. 2011 Jun;23(6):2348 - 2361.

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Marion Avril., et al. From The Cover: Pnas Plus: A Restricted Subset Of 亚博的官网地址 Genes Mediates Adherence Of Plasmodium Falciparum-Infected Erythrocytes To Brain Endothelial Cells. Proc Natl Acad Sci U S A. 2012 Jun;109(26):E1782 - E1790.

Shanmugabalaji V., et al. Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants. Plant Mol Biol. 2013 Jan;81(1-2):13-25.

Deqiang Duanmu., et al. Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival. Proc Natl Acad Sci U S A. 2013 Feb;110(9):3621-6.

Piuri M., et al. Generation of Affinity-Tagged Fluoromycobacteriophages by Mixed Assembly of Phage Capsids. Appl Environ Microbiol. 2013 Sep;79(18):5608-15.

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产品编号: A01732
规格
价格
3320 A01732-100 100 ug ¥2500